FICUS CARICA L. EXTRACT: A BEACON OF HOPE FOR AGS HUMAN GASTRIC ADENOCARCINOMA
Authors: Kwame Osei, C. Adjei E.
Published: March 2024
Abstract
<p><strong><em>Objective: </em></strong>Gastric cancer is one of the gastrointestinal tract tumors associated with a poor prognosis. <em>Ficus carica </em>L. is one of the most outstanding species among many traditional medicines. The aim of our study is to determine the antioxidant content of <em>Ficus carica </em>L., and its therapeutic effects on gastric cancer.</p> <p><strong><em>Patients and Methods: </em></strong><em>Ficus carica </em>L. was extracted in a different solvent. The antioxidant parameters of the different concentrations of extracts were measured photometrically. The human gastric adenocarcinoma cells (AGS) were incubated with different concentrations of <em>Ficus carica </em>L. extract for 24 hours; cell viability by the ATP test, and intracellular reactive oxygen species levels were determined by H2DCF-DA fluorescent dye. Intracellular glutathione levels luminometrically, and mitochondrial membrane potential fluorometrically were detected. The apoptotic ef-</p> <p>fect was analyzed with acridine orange/ ethidium bromide, and the genotoxic effect by the comet assay.</p> <p><strong><em>Results: </em></strong>It was found that methanol extract has higher antioxidant content compared to ethanol extract (<em>p</em><0.001). In addition, it has been revealed that methanol extract has cytotoxic, genotoxic, and apoptotic effects on gastric cancer cells statistically significantly (<em>p</em><0.001).</p> <p><strong><em>Conclusions: </em></strong>As a result of our study, it is thought that <em>Ficus carica </em>L., which has an anticancer effect, can be used together with routine treatments in gastric cancer.</p>
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